Facile and Unplugged Surface Plasmon Resonance Biosensor with NIR-Emitting Perovskite Nanocomposites for Fast Detection of SARS-CoV-2

Anal. Chem., 95 (2023).

https://pubs.acs.org/doi/10.1021/acs.analchem.2c05661

我們利用3D列印技術開發了一種基於光譜式表面電漿共振(SPR)的便攜式生物感測平台，並合成了穩定於空氣中的近紅外鈣鈦礦量子點作為光源。鈣鈦礦量子點的簡單合成過程使得低成本、大面積生產和良好的發光穩定性成為可能。結合自製的高親和力抗體，此平台能夠區分來自COVID-19患者和健康受試者的臨床檢體，最重要的是，整個測量過程不僅耗時不到15分鐘，而且無需複雜的操作或多種試劑。這些尖端技術的無縫整合使得SPR感測器具有輕巧、緊湊且無須插頭等特點，非常適合用於現場檢測高傳染性疾病。

Our research team has successfully developed a portable biosensing platform based on wavelength-interrogated surface plasmon resonance (SPR), utilizing 3D printing technology and incorporating air-stable, NIR-emitting perovskite quantum dots as the light source. The streamlined synthesis process for the perovskite quantum dots facilitates cost-effective, large-scale production and ensures reliable emission stability. In conjunction with a custom-developed, high-affinity polyclonal antibody, our platform has demonstrated the capability to accurately discriminate between clinical swab samples collected from COVID-19 patients and healthy individuals. Notably, the entire measurement process requires less than 15 minutes to complete and eliminates the need for intricate procedures or multiple reagents. The seamless integration of these cutting-edge technologies has resulted in an SPR sensor that boasts a lightweight, compact design without the need for a plug, rendering it exceptionally well-suited for on-site detection of highly transmissible disease.